ABSTRACT
Objective To study the effect of Semen Euphorbiae and Semen Euphorbiae Pulveratun on the microflora in rats gastrointestinal from the aspect of the intestinal microecology, so as to provide reference for further revealing mechanism of the attenuation after processing of Semen Euphorbiae. Methods Rats were fed with high, medium and low dose of Semen Euphorbiae and Semen Euphorbiae Pulveratun, and the plate-counter method was used to examine the four types of intestinal flora in the feces of rats which are Bifidobacterium, Lactobacillus, Escherichia coli, and Enterococcus. Results After intragastric administration, the rat had the alteration of intestinal flora, and the degree of alteration of intestinal flora in the Semen Euphorbiae Pulveratun group was lower than the group of Semen Euphorbiae. In the low doses of group, the number of Enterococci and Escherichia coli was decreased. Conclusion Semen Euphorbiae and Semen Euphorbiae Pulveratun could affect the intestinal microbial diversity and the balance of intestinal flora. The effect of Semen Euphorbiae Pulveratun on four kinds of intestinal flora was weakened, and the degree of intestinal flora disturbance was reduced, which was consistent with the results of alleviation of the effect of Semen Euphorbiae Pulveratun. It was suggested that the intervention effect of Semen Euphorbiae on intestinal microflora could be clarified from the perspective of intestinal microecology, which could reveal possible correlation between Semen Euphorbiae Pulveratun and the changes of intestinal flora.
ABSTRACT
Study on the standardization of Chinese materia medica is an important action for modernization and globalization for traditional Chinese medicine. Standardization on the processing of Chinese herbal pieces is an important part in the study on standardization of Chinese materia medica, so it is of great significance to establish the technical processing standards of Angelicae Sinensis Radix pieces for improving its quality. In this study, single factor experiment was designed to optimize the softening, cutting and drying processes of Angelicae Sinensis Radix. With ferulic acid, Angelicae Sinensis Radix polysaccharide, volatile oil and extracts (water and ethanol) content as the quality index, the effects of different softening, cutting and drying processes on the contents of the five components in Angelicae Sinensis Radix were analyzed, and the normalized distance evaluation method was used to analyze the experimental data. The results showed that the content of five components in Angelicae Sinensis Radix was affected by different softening methods and drying temperature, but the thickness of slice had little effect on the content. The best preparation process for Angelicae Sinensis Radix was as follows: Non-medicinal parts were removed; mildewed and rot as well as moth-eaten parts were removed; washed by the flowing drinking water; stacked in the drug pool; moistening method was used for softening, where 125 mL water was sprayed for every 1 kg of herbs every 2.5 h; upper part of herbs covered with clean and moist cotton, and cut into thin slices (1-2 mm) after 15 h moistening until appropriate softness, with disk thickness of 1-2 cm, then received blast drying for 6 h at 55 ℃, and turned over for 2 times during the drying.
ABSTRACT
Notopterol, isoimperatorin, volatile oil and extract （water and ethanol) were used as the research objects in this study to investigate the effects of different softening method, slice thickness and drying methods on the quality of Notopterygii Rhizoma et Radix slices, and the experimental data were analyzed by homogeneous distance evaluation method. The results showed that different softening, cutting and drying processes could affect the content of five components in Notopterygii Rhizoma et Radix incisum. The best processing technology of Notopterygii Rhizoma et Radix slices was as follows: non-medicinal parts were removed; mildewed and rot as well as moth-eaten parts were removed; washed by the flowing drinking water; stacked in the drug pool; moistening method was used for softening, where 1/8 volume of water was sprayed for every 1 kg of herbs every 2 h; upper part of herbs covered with clean and moist cotton, and cut into thick slices (2-4 mm) after 12 h moistening until appropriate softness, then received blast drying for 4 h at 50 ℃, and turned over for 2 times during the drying. The process is practical and provides the experimental basis for the standardization of the processing of Notopterygii Rhizoma et Radix, with great significance to improve the quality of Notopterygii Rhizoma et Radix slices.
ABSTRACT
<p><b>OBJECTIVE</b>To detect the expression of TFPI-2 gene in pancreatic carcinoma, and to evaluate its prognostic significance in patients with pancreatic carcinoma.</p><p><b>METHODS</b>Reverse transcription polymerase chain reaction (RT-PCR) and Western blot were used to detect the expression of TFPI-2 mRNA and protein in the pancreatic carcinoma tissue samples from 41 patients. The correlation of its expression with clinicopathological features and its prognostic significance were analyzed.</p><p><b>RESULTS</b>The expression of TFPI-2 mRNA and protein in moderately or poorly differentiated pancreatic carcinoma tissues, or in cases with nerve-involvement, lymph node and blood vessel invasion was significantly lower than that in the highly differentiated one, or without nerve involvement, or neither lymphatic nor blood vessel invasion (P < 0.05). The median survival time of patients with high expression of TFPI-2 (12.0 months) was significantly longer than that with low expression of TFPI-2 (5.1 months, P < 0.05). The Cox model analysis showed that the expression of TFPI-2 was an independent marker for prognosis in patients with pancreatic carcinoma.</p><p><b>CONCLUSION</b>The expression of TFPI-2 is correlated with clinical stage and differentiation of pancreatic carcinoma, and can be used as a prognostic marker for pancreatic carcinoma.</p>